FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002540251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16550575
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4819882.9122130197893425No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT3202241.9348209956451663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2309621.3954923016269827TruSeq Adapter, Index 5 (100% over 50bp)
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG2056031.2422710389216085No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG1238510.748318411898076No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG1209940.7310561717644252No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC1135150.6858674094404574No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA1011870.6113805713698769No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC1009700.6100694386750913No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC1000500.6045107194160928No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG989760.5980215188898271No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG972790.5877680986914352No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC901970.5449780445694485No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT857740.5182538975231978No Hit
GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA839210.5070579118852366No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG813720.49165663428611994No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT767580.46377844878501207No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA749560.4528906095407561No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT737580.44565219033175585No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC737450.4455736432117917No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA698570.4220820122563718No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG687940.41565927467776803No Hit
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGT658160.3976659421198357No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA656340.39656628244033815No Hit
GCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATT629840.38055475413996187No Hit
GCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCC629150.38013785019553703No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC604310.3651293081962409No Hit
CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT602460.3640115222582901No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT593020.35830779293166554No Hit
GCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCT575310.3476072583580933No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT570220.3445318365071908No Hit
GCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACAC564200.34089450064423743No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG562400.3398069251370421No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA512390.30959045229546406No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG503880.30444863698089036No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT500680.30251516941254303No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG476980.2881954252344707No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA469460.28365177644885453No Hit
GTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447550.2704135656918264No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC445760.2693320322707821No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC438340.26484880434667674No Hit
CAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTG423330.2557796330338976No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA419180.25327216728119717No Hit
GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC412710.24936293754144492No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA410340.2479309631236377No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA408680.24692797682255752No Hit
GTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGT401360.242505169759963No Hit
CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC400150.24177407733568168No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG394350.23826966736805216No Hit
ACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTC386330.23342391427488168No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC378170.22849357197559603No Hit
GTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA372800.22524897171246316No Hit
GTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAG371450.22443329008206664No Hit
GGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGAT369510.22326112536875609No Hit
CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC367420.2219983293631792No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT364720.22036696610238615No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC358700.21672963023943279No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT355170.2145967738280996No Hit
CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC354110.21395631269608456No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA347880.2101920930239584No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG346090.2091105596029141No Hit
GCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAG345460.20872990817539572No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA321900.19449475320343856No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA320140.19343134604084752No Hit
CCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA317480.1918241511246588No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA315930.19088762777124058No Hit
CACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAAT313710.18954628464569961No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA309190.18681526170540902No Hit
CGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGA308460.1863741894163798No Hit
GGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTC304760.1841386175404782No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC302760.1829302003102611No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC298220.18018709319766837No Hit
GTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCC297680.17986082054550975No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG297640.17983665220090542No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAA297460.17972789465018588No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA296950.17941974825648052No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG293780.17750440694658645No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA292460.17670685157464316No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG288240.17415709121888515No Hit
GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA282810.17087623843884578No Hit
GCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGG279090.16862858239064202No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA277160.16746245976348254No Hit
CCCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT276270.16692471409603593No Hit
GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTA268740.16237502322426864No Hit
CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAG264350.15972254740394215No Hit
GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT262990.15890082368739455No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC261700.15812139457390453No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC256910.15522723530753463No Hit
GGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG256630.15505805689530425No Hit
CCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC255280.15424237526490772No Hit
GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA246950.1492093175010536No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA246420.14888908693504607No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG245300.1482123732861245No Hit
GCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGC244230.14756587006795838No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTT244040.14745107043108774No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG242300.1463997474407989No Hit
CCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGT240950.14558406581040237No Hit
CCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA237800.14368080867281047No Hit
GGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTG234140.1414694051415132No Hit
GTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCA233800.1412639742123763No Hit
CGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTA230440.13923383326561162No Hit
CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC230250.13911903362874098No Hit
GATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCT227530.13747558619564576No Hit
CCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT222370.1343578697416857No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA219430.1325814964132666No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA212740.1285393407781905No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC212660.12849100408898181No Hit
GCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT211140.12757260699401682No Hit
CTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGC211080.12753635447711031No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG210070.12692610377585067No Hit
GCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGA208790.12615271674851175No Hit
GCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACAT208030.12569351820102928No Hit
GGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCCGT204540.12358483013430047No Hit
GCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACC201580.12179637263357919No Hit
CCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAA196830.11892638171181365No Hit
ACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAG191780.11587512820551553No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC187470.11327098907439773No Hit
CCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTAC186160.11247947578860552No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTC185900.11232238154867732No Hit
GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT185800.11226196068716646No Hit
CCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGA184660.11157316286594272No Hit
TTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAG184600.1115369103490362No Hit
AACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGG183550.11090249130317224No Hit
GGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAG182960.11054600822025822No Hit
CCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACA182740.11041308232493433No Hit
GTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCG181450.1096336532114443No Hit
GGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTAC181420.10961552695299107No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC181200.10948260105766719No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA179380.10838294137816963No Hit
CCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAG179200.10827418382745009No Hit
GCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCC179050.10818355253518382No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT178410.10779685902151435No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178280.10771831190155025No Hit
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT177270.10710806120029064No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGG175280.10590568605622463No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGA172290.10409910229705009No Hit
GCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGC171990.10391783971251754No Hit
GTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCA171740.10376678755874041No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT170640.10310215808212102No Hit
GCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT170520.103029653048308No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC167840.1014103739598171No Hit
ATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTC166410.10054635564021189No Hit
GTCGGTTTGGGGTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCT166220.10043155600334126No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG165910.10024425133265762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)