FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002540261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18336128
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC4189892.2850462213178266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3253801.7745294971762848No Hit
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCT1909211.0412285516331474No Hit
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCG1835811.0011982900642928No Hit
GCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC1823690.9945883885627326No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT1464050.7984510143035651No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG1075840.5867323788315614No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGC1053900.5747669300737865No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT903180.49256855100487956No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC816960.4455466279467508No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG737940.40245137904796474No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG714220.38951516917857465No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC695100.37908766779987574No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC694250.3786241021005089No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC691110.37691163586990667No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC690820.3767534781607109No Hit
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG690390.37651896845397237No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC669740.36525704881641313No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCC644200.351328262978967No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA610130.3327474590055218No Hit
GGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG594830.3244032764169185No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC572450.31219786423829504No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG541500.29531861906723167No Hit
CGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC541020.29505684078994215No Hit
CCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC537550.29316440199370336No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA511030.27870115217345776No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT508230.2771741122226023No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCC506230.27608336940056266No Hit
GGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG503660.27468176487424173No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT501310.2734001420583452No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA494730.2698115981738347No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG490840.2676901033849676No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT456310.2488584285624533No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT438420.2391017340193088No Hit
CCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCG408210.22262606369240004No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG402140.21931565922750973No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG401380.2189011769551347No Hit
CCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCG373070.20346171230916366No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA363880.1984497490418915No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA360450.19657912510209352No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG358980.1957774291278944No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG344740.18801134023497218No Hit
CCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAG342070.18655519856754926No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC336350.1834356740965159No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA335250.1828357655443941No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG334500.18242673698612924No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA326770.17821101597894604No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG321130.17513512122079428No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC319270.17412073039629739No Hit
CGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACG315780.17221738417183824No Hit
GTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGG307940.16794167230944287No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT306760.16729813404443947No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCC300520.1638950164396758No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA296590.16175170679436793No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT281000.15324936649656895No Hit
GGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCT279490.15242585566592903No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA269350.14689578955818808No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA265680.14489427647974534No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA262800.14332360681600825No Hit
CCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGG258240.1408367131817579No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG257840.14061856461734995No Hit
CGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGA256180.13971324807505706No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA255030.13908607095238426No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC250810.13678460359788064No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG250230.13646828817948917No Hit
GGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGC243960.1330488094323949No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG243420.13275430887044418No Hit
GACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGG240820.13133634320179266No Hit
CACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAAT239690.13072007350734027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC237900.12974385868161478TruSeq Adapter, Index 12 (100% over 50bp)
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCCCG237290.1294111821208927No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA232930.12703336276884628No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA225850.12317213317882597No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG222870.12154692637398692No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC220900.12047254469427789No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCCC220330.12016168298999658No Hit
CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT218940.11940361672867904No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG214200.11681855624044508No Hit
GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA210710.11491521001598592No Hit
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGT205040.11182295411550355No Hit
CGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGG193760.10567116459920001No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG192050.1047385794863561No Hit
GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC191930.10467313491703374No Hit
GCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCC191120.10423138407410767No Hit
CCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGAC188600.10285704811833773No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT188360.10272615897969298No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC186300.10160269387299217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)