FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002540262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30936747
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1153460.3728446303678923TruSeq Adapter, Index 5 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT412180.13323314180382315No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC407290.1316524972712871No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA377420.12199731277499862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)