FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002540267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26333379
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC3846401.460655694812276No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2054370.7801391534295694TruSeq Adapter, Index 4 (100% over 50bp)
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG1575490.5982863042376749No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC864770.3283931013942419No Hit
CCGCGGGGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCT746410.2834463438968467No Hit
GGGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTC693530.26336536606259303No Hit
CCCGCGGGGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGC619850.2353856677489053No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA597610.22694011277474116No Hit
GTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGC460390.1748313423810898No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG442580.16806806297057433No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC406240.15426808690217841No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG399380.15166302812867274No Hit
CGCGGGGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTC398710.1514085981901525No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA381900.14502506495653292No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA374530.14222633563281034No Hit
CCTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTG355310.13492761411287174No Hit
GGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCG355230.13489723441872006No Hit
GCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCG341170.1295580031715641No Hit
CCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGC340970.12948205393618495No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA339760.12902256106214094No Hit
GGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCC336700.12786053776083958No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT334020.12684281800675865No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA331160.12575674394083644No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA317070.12040612030837364No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC316570.1202162472199257No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG310670.11797574477624008No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG295590.11224917242865033No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC283710.1077377878471274No Hit
GGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCG283130.10751753506452781No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG274240.1041415915519235No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTT269690.1024137464470473No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA264010.10025678816227876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)