FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002540269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531226
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC2736111.476486229243548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1593210.8597434406120782No Hit
GCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC1590870.8584807071048618No Hit
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCG1411620.7617520826738609No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1389030.749561847661887TruSeq Adapter, Index 13 (97% over 40bp)
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCT1128650.6090530653503443No Hit
GGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG1115740.6020864458724965No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCC1034340.5581605879718913No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG977940.5277254726697521No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT870850.4699365276749633No Hit
GGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG841460.45407681067620675No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGC826580.4460471206815998No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCC746380.402768818425721No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC562270.3034175936335783No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCC551950.2978486150889315No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG543890.29349919967518606No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC542400.2926951514163175No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA530150.2860846875430692No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC519820.28051031270138305No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT507220.2737109784317562No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA500160.2699011927219494No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA463390.2500590085081257No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC450080.24287653714870241No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT448780.24217501853358217No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC441580.238289684665224No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG434320.23437197301462948No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG433570.2339672507366755No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC419330.22628292375258927No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC412860.2227915195681063No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA387240.2089662065531984No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG378520.2042606355348534No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA374760.20223162784804416No Hit
CCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC367710.19842723843527676No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC364430.19665725300635806No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG347830.18769939992097665No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC347430.18748354803940118No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG347430.18748354803940118No Hit
GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC337780.18227612139639332No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC327020.17646970578201357No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT323080.17434356474849533No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG320660.1730376608649638No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT315310.1701506419488921No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA312730.1687583973127304No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA310700.16766294901373496No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT310350.16747407861735644No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT308550.16650274515026692No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA293870.1585809810964477No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG293460.15835973291783284No Hit
CGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC286800.15476579908960153No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG285160.15388080637514215No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA275480.1486571908410161No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG269640.14550575337001448No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG261360.14103761942140255No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA260650.14065448233160613No Hit
CCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCG259100.13981805629050123No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT249620.13470236669716293No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA247690.13366088136856136No Hit
GGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGC239710.12935463633113103No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT237160.12797858058608752No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC222450.12004062764115013No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG220440.11895597193623346No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG216380.1167650753382426No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA212150.11448244169058215No Hit
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG212150.11448244169058215No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG210990.11385647123401334No Hit
CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT204330.110262537405782No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA201840.10891885944297479No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC199720.10777484447062487No Hit
CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC195980.10575662937789437No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT195570.10553538119927952No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG189600.10231379186676585No Hit
CCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCG188840.10190367329177248No Hit
GGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGAC188800.10188208810361496No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC187760.10132087321151878No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA186650.10072188424014687No Hit
GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT185460.10007972489245989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)