FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002540277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35528344
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1305680.3675037598149804TruSeq Adapter, Index 14 (97% over 44bp)
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT681410.19179334674309617No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC596810.16798137284417197No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT581130.163567995175908No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT533880.1502687544344876No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG482890.13591683305025418No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG435620.12261196300058343No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC433530.12202370028842324No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG363680.10236334122412236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)