FastQCFastQC Report
Wed 8 May 2024
EGAF00002542466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62624861
Total Bases500.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGACC1704421227.21636699520978No Hit
ATCTCTGT1624723125.943739819238882No Hit
GGAGAGAG1356239221.65656223971499No Hit
TAGCTCTA1087173917.360100807249694No Hit
NCTAGACC5732850.9154271815469579No Hit
NTCTCTGT4973490.7941718225929475No Hit
NGAGAGAG4520840.7218922210462072No Hit
NAGCTCTA3453730.5514950364520569No Hit
ATCGCTGT2361380.3770675035909461No Hit
TAGCGCTA1915560.30587852322738085No Hit
TAGCCCTA1738020.2775287597045525No Hit
ATCTCTGG1642190.2622265301315399No Hit
ATCCCTGT1435360.22919971031951672No Hit
ATCTCGGT1414510.22587036161245932Illumina Single End PCR Primer 1 (100% over 8bp)
ATCACTGT1123200.17935369149961067Illumina PCR Primer Index 5 (100% over 8bp)
CCGAGACC1082360.1728323197395999Illumina Paired End PCR Primer 2 (100% over 8bp)
ATCTCTGA957720.1529296807541018No Hit
TAGCACTA926590.1479588114375216No Hit
GAGCTCTA904740.14446978173731995No Hit
AGCTCTGT867570.13853443922214853No Hit
ATCTCTGC803090.1282382087842079No Hit
CCCAGACC695820.11110922864962526No Hit
ATCTCCGT686210.10957469430550912No Hit
GGAGAGGG663430.10593716128168332No Hit
TAGCTCGA648760.10359464111225732No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)