FastQCFastQC Report
Wed 8 May 2024
EGAF00002542467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63687905
Total Bases509.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGACC1728141827.13453676957972No Hit
ATCTCTGT1662781426.108275974849544No Hit
GGAGAGAG1369915821.50982670885469No Hit
TAGCTCTA1112110317.461876002986752No Hit
NCTAGACC6450281.0127951296246909No Hit
NTCTCTGT5998340.9418334611571852No Hit
NGAGAGAG5080430.7977071941681864No Hit
NAGCTCTA4081890.6409207525353519No Hit
ATCGCTGT1987980.31214404053642525No Hit
TAGCGCTA1710750.2686145823135492No Hit
ATCTCTGG1628880.2557597082208938No Hit
TAGCCCTA1545210.24262220589608027No Hit
ATCTCGGT1302670.204539621769628Illumina Single End PCR Primer 1 (100% over 8bp)
ATCCCTGT1268780.19921836022083No Hit
ATCTCTGA977370.15346241959128662No Hit
CCGAGACC946960.14868757262466084Illumina Paired End PCR Primer 2 (100% over 8bp)
ATCACTGT876540.13763052811989968Illumina PCR Primer Index 5 (100% over 8bp)
AGCTCTGT864770.1357824535129551No Hit
TAGCACTA835400.13117090285824287No Hit
ATCTCTGC810370.1272408002744006No Hit
GAGCTCTA661160.10381248998534338No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)