FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002542472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62507706
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTAT1667259926.672869741852306No Hit
GGTTCCTC1462338623.394533147641027ABI Solid3 Adapter A (100% over 8bp)
ATCATGCA1458536123.3337006480449No Hit
TAACAAGG995181415.920939411854276No Hit
NCGGGTAT5344370.8549937826865698No Hit
TACCAAGG5109480.8174160158749068No Hit
NTCATGCA4747520.7595095555098438No Hit
NGTTCCTC4416380.7065336872225002No Hit
GGGTCCTC4011610.6417784712816048No Hit
CCGGGGAT3584350.5734252989543401No Hit
NAACAAGG3457590.5531461992862128No Hit
GGATCCTC2924460.4678559152370749No Hit
CCGGGAAT2405950.38490454280948977No Hit
GGCTCCTC2274140.3638175427522488No Hit
AGCATGCA2098330.33569141059183966No Hit
CCGGGCAT1915260.30640382163440777No Hit
CCGGGTAG1886870.3018619816251135No Hit
CCGGGTAC1795870.2873037765935611No Hit
GGTACCTC1760130.28158608156248766ABI Dynabead EcoP Oligo (100% over 8bp)
GGTTCCGC1545350.2472255180825225No Hit
GAACAAGG1543830.24698234806441302No Hit
CCGGGTAA1465570.23446229173727795No Hit
GGTGCCTC1377710.22040642476945163No Hit
GGTTCCCC1012610.1619976263406627No Hit
ACCATGCA977400.15636472085537742No Hit
AAACAAGG949820.15195246486889152No Hit
AACATGCA905800.14491013316022186No Hit
GGTCCCTC800950.12813620131892217No Hit
GGTTGCTC712900.11404993809883217No Hit
CAACAAGG669130.10704760145893051No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)