FastQCFastQC Report
Wed 8 May 2024
EGAF00002542473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63700489
Total Bases509.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTAT1702697826.729744570720644No Hit
GGTTCCTC1500799723.560253988003137ABI Solid3 Adapter A (100% over 8bp)
ATCATGCA1480254823.23773056122065No Hit
TAACAAGG1011408615.877564142403994No Hit
NCGGGTAT6233960.9786361294651914No Hit
NTCATGCA5485650.8611629339297537No Hit
NGTTCCTC5354560.8405838140426206No Hit
TACCAAGG5164070.8106798049854844No Hit
NAACAAGG3878140.6088085132282108No Hit
GGGTCCTC3637220.5709877674565418No Hit
CCGGGGAT3382970.5310744160849378No Hit
GGATCCTC2968270.4659728750276941No Hit
CCGGGAAT2272510.356749223699052No Hit
GGCTCCTC2246830.35271785747202034No Hit
AGCATGCA2021290.31731153586591776No Hit
CCGGGCAT1885200.2959474926479764No Hit
CCGGGTAG1846020.28979683342776225No Hit
CCGGGTAC1747600.2743464025841309No Hit
GGTACCTC1530640.2402870094136954ABI Dynabead EcoP Oligo (100% over 8bp)
CCGGGTAA1436590.22552260156119053No Hit
GGTTCCGC1301290.20428257622951687No Hit
GAACAAGG1236410.19409741108894785No Hit
GGTGCCTC1177230.18480705854550034No Hit
ACCATGCA968050.15196900607780264No Hit
AACATGCA915380.1437006237110676No Hit
AAACAAGG870840.1367085266802269No Hit
GGTTCCCC866830.1360790181689186No Hit
GGTCCCTC731420.11482172452396716No Hit
GGTTGCTC648840.10185793079233663No Hit
CAACAAGG643120.10095997850189187No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)