FastQCFastQC Report
Wed 8 May 2024
EGAF00002542478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56427745
Total Bases451.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCGT2175864538.56018878656236No Hit
ACAAGGTA1392408624.67595683648177No Hit
GTGGTACC934772016.565822362740175No Hit
TACTATAG617107510.93624244598114No Hit
NGTCCCGT6974051.2359256957725318No Hit
CGTCCCGG5171340.9164534219823954No Hit
NCAAGGTA4561050.8082991797740633No Hit
ACAAGGGA3242040.5745471487474823No Hit
GGGGTACC3066850.5435003649357244No Hit
NTGGTACC2964480.525358580251612No Hit
CGTCCCGC2694390.4774938286121482No Hit
CGTCCCGA2629600.4660118883006932No Hit
CGGCCCGT2446350.43353672913918495No Hit
NACTATAG2017680.35756878110227514No Hit
ACAAGGAA1529570.27106700790541954No Hit
ACAAGGCA1484490.2630780301427959No Hit
GAGGTACC1453250.2575417465291232ABI Dynabead EcoP Oligo (100% over 8bp)
GCGGTACC1249020.2213485582314161No Hit
CGCCCCGT953290.16893994257612102No Hit
TACGATAG747850.13253232075816604No Hit
GACTATAG609100.10794335304379078No Hit
GTGGGACC581030.10296884980961052No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)