FastQCFastQC Report
Wed 8 May 2024
EGAF00002542479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57384864
Total Bases459 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCGT2216174938.619502522477006No Hit
ACAAGGTA1416708124.687835802834698No Hit
GTGGTACC951353016.578465708309427No Hit
TACTATAG628866010.958743406623741No Hit
NGTCCCGT8131521.4170147723971256No Hit
NCAAGGTA5223500.9102574504663807No Hit
CGTCCCGG4894430.8529130608377847No Hit
NTGGTACC3476920.6058949621279925No Hit
ACAAGGGA2934750.5114153446455846No Hit
GGGGTACC2862650.4988510559160687No Hit
CGTCCCGC2584780.45042887964324535No Hit
CGTCCCGA2544150.4433486154118968No Hit
NACTATAG2349360.40940412440465135No Hit
CGGCCCGT2210310.3851729961405851No Hit
GAGGTACC1419510.24736662266900208ABI Dynabead EcoP Oligo (100% over 8bp)
ACAAGGAA1406100.24502976952249989No Hit
ACAAGGCA1391170.24242803816699818No Hit
GCGGTACC1199530.20903247239550835No Hit
CGCCCCGT869700.15155564366241245No Hit
TACGATAG638010.11118088560774493No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)