FastQCFastQC Report
Wed 8 May 2024
EGAF00002542484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56833000
Total Bases454.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC1953502434.37267784561786No Hit
TTTACATG1156291120.345417275174636No Hit
GAATTCCA1071130318.846977988140694Illumina Small RNA Adapter 2 (100% over 8bp)
ACGCGGGT1011264717.793618144387942No Hit
NGCGATAC6519661.1471609804163074No Hit
ACGCGGGG4041780.7111678074358208No Hit
NTTACATG3779310.6649851318776063No Hit
NAATTCCA3337410.5872310101525522No Hit
NCGCGGGT3278420.5768514771347633No Hit
ACGCGGGA2595500.4566888955360442No Hit
ACGCGGGC2386880.41998134886421623No Hit
CGCGAGAC2001060.35209473369345273No Hit
GTTACATG1658950.29189907272183413No Hit
CGCGAAAC1438430.25309767212710926No Hit
GAAGTCCA1207790.21251561592736615No Hit
TTTACAGG1161430.20435838333362658No Hit
TGTACATG1095210.19270670209209437No Hit
CGCGACAC1075510.18924040610208856No Hit
GAAATCCA1063350.18710080411028804No Hit
ATTACATG804510.14155684197561275No Hit
GAATGCCA789390.1388964158147555No Hit
GAATCCCA664030.11683880843875918No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)