FastQCFastQC Report
Wed 8 May 2024
EGAF00002542485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57612409
Total Bases460.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC1974638634.274536237497024No Hit
TTTACATG1180291520.48675833013683No Hit
GAATTCCA1088104718.886637772775654Illumina Small RNA Adapter 2 (100% over 8bp)
ACGCGGGT1025391417.798099711470144No Hit
NGCGATAC7325611.2715333601134435No Hit
NTTACATG4420580.7672965037792465No Hit
NAATTCCA3964190.6880791948831718No Hit
ACGCGGGG3780830.6562527180559313No Hit
NCGCGGGT3758150.6523160661447086No Hit
ACGCGGGA2456790.42643417323514454No Hit
ACGCGGGC2304310.39996765280202046No Hit
CGCGAGAC1834130.3183567623426405No Hit
CGCGAAAC1290500.22399688233831708No Hit
GTTACATG1254300.21771351376749407No Hit
TGTACATG1045450.1814626428830636No Hit
GAAGTCCA1030530.17887292301906693No Hit
CGCGACAC1014300.17605582158524216No Hit
TTTACAGG1010660.17542401325381135No Hit
GAAATCCA889130.15432959937502355No Hit
ATTACATG690910.11992381710683198No Hit
GAATGCCA690470.11984744467116451No Hit
GAATCCCA624320.10836554326343131No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)