FastQCFastQC Report
Wed 8 May 2024
EGAF00002542490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63759374
Total Bases510 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATGC1755973527.540632691908172No Hit
CAGCCACT1725869827.06848721569945No Hit
ACTAGGAG1271002819.934367611576615No Hit
TGATTCTA1044330316.37924330938381No Hit
NTCGATGC5684580.8915677246141093No Hit
NAGCCACT5616960.8809622252564776No Hit
GGCGATGC4348830.6820691181817438No Hit
NCTAGGAG4184200.6562486011860782No Hit
CAGCCACC3446840.5406012926036571No Hit
CAGCCACG3414570.5355400760365057No Hit
NGATTCTA3295330.5168385122476266No Hit
CAGCCACA2399840.3763901446083834No Hit
GCCGATGC2228680.3495454644833872No Hit
ACCAGGAG1986210.31151654657086186No Hit
GGATTCTA1871420.2935129193708834No Hit
ACGAGGAG1760480.2761131249500661No Hit
GACGATGC1669740.2618814921238091No Hit
ACAAGGAG1194020.18726971817508747No Hit
TGAGTCTA1183300.18558839677441No Hit
TGAATCTA1021330.16018507333525578No Hit
GTCGAGGC919910.1442783926956372No Hit
TGATGCTA845610.13262520425624005No Hit
AGATTCTA780840.12246669799487052No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)