FastQCFastQC Report
Wed 8 May 2024
EGAF00002542491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64884143
Total Bases519 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATGC1785179327.51333711843894No Hit
CAGCCACT1756925027.077879413464707No Hit
ACTAGGAG1292358419.917938963916036No Hit
TGATTCTA1072591316.530869491487312No Hit
NTCGATGC6583121.0145961240483674No Hit
NAGCCACT6483320.9992148620965834No Hit
NCTAGGAG4793360.7387567714348943No Hit
GGCGATGC4044560.6233510705381436No Hit
NGATTCTA3961180.6105004731279259No Hit
CAGCCACC3256320.5018668428740748No Hit
CAGCCACG3249530.500820362226253No Hit
CAGCCACA2317800.3572213321828108No Hit
GCCGATGC2147980.3310485275269799No Hit
ACCAGGAG1814740.2796892917272561No Hit
GACGATGC1663110.2563199455373865No Hit
ACGAGGAG1551250.23907998599904445No Hit
GGATTCTA1458430.22477448765871808No Hit
ACAAGGAG1122820.1730499854178547No Hit
TGAGTCTA1027580.15837151459332674No Hit
TGAATCTA860440.13261175384561988No Hit
GTCGAGGC824460.1270664852581932No Hit
TGATGCTA767450.11828005495888264No Hit
AGATTCTA689880.1063248997524711No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)