FastQCFastQC Report
Wed 8 May 2024
EGAF00002542496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61578623
Total Bases492.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTTA1543792925.07027316931072No Hit
TAATGACC1455091923.629821991959773No Hit
GCCGAGAT1382606522.452702458124143No Hit
CGTATCGG1176834319.111085027023094No Hit
NTGCCTTA5499600.8931021403320435No Hit
NAATGACC5426760.8812733600749727No Hit
NCCGAGAT4962550.805888433068729No Hit
NGTATCGG4244210.6892343143171616No Hit
GCCGAGAG3645320.5919781609926549No Hit
ATGCCGTA2791590.45333751617018136No Hit
GCCGAGAA2273880.36926450921125664No Hit
GCCGAGAC1953550.31724483348710153Illumina Paired End PCR Primer 2 (100% over 8bp)
GAATGACC1874550.30441570608033897No Hit
AAATGACC1608780.2612562479677404No Hit
CGTAGCGG1567410.25453800745105976No Hit
ATGCCCTA1532300.24883635348585173No Hit
ATGCCATA1257670.20423808437548208No Hit
CGGATCGG1078550.1751500679058705No Hit
CGTAACGG967120.157054502501623No Hit
CGTACCGG946270.15366858723034452No Hit
TAAGGACC919550.14932941907453826No Hit
ATGCCTAA846460.13746004031301576Illumina PCR Primer Index 3 (100% over 8bp)
ATGCCTGA844390.13712388469615502No Hit
CGCATCGG842200.13676824179715744No Hit
CGAATCGG784150.12734126906345405No Hit
CAATGACC764380.12413073933140725No Hit
AGGCCTTA700090.11369042792658746No Hit
TAAAGACC694870.11284273115363427No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)