FastQCFastQC Report
Wed 8 May 2024
EGAF00002542497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61947441
Total Bases495.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTTA1570728925.35583188981124No Hit
TAATGACC1474692423.805541862495982No Hit
GCCGAGAT1398696822.578766409414715No Hit
CGTATCGG1191473619.23362096587654No Hit
NTGCCTTA5815830.9388329697105648No Hit
NAATGACC5668240.9150079339031938No Hit
NCCGAGAT5187770.837447022226471No Hit
NGTATCGG4449580.7182831006691625No Hit
GCCGAGAG3702440.597674405953266No Hit
GCCGAGAA2253540.3637825814306034No Hit
ATGCCGTA2205700.3560599056868225No Hit
GCCGAGAC1981200.31981950634571005Illumina Paired End PCR Primer 2 (100% over 8bp)
GAATGACC1848920.29846592048895126No Hit
AAATGACC1554310.250907862360287No Hit
CGTAGCGG1500020.24214398137931153No Hit
CGGATCGG1141230.18422552757264016No Hit
ATGCCCTA1120650.18090335644373107No Hit
CGTACCGG926280.1495267576912499No Hit
CGTAACGG905580.14618521530211395No Hit
CGCATCGG894550.14440467363292697No Hit
CGAATCGG835060.1348013713754536No Hit
ATGCCTAA832840.1344430030612564Illumina PCR Primer Index 3 (100% over 8bp)
ATGCCATA818930.13219755114662443No Hit
ATGCCTGA814410.13146790034474548No Hit
CAATGACC776540.12535465347148078No Hit
AGGCCTTA644300.10400752470146425No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)