FastQCFastQC Report
Wed 8 May 2024
EGAF00002542502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63020740
Total Bases504.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGAC1578383625.045462811131703No Hit
GCAGTAGA1530175624.280508289810623No Hit
CAGTACTG1383125121.947141528328608No Hit
AGTAGTCT1253180419.885206044867136No Hit
NTCCCGAC5828280.9248193531208933No Hit
NCAGTAGA5545570.8799595180888069No Hit
NAGTACTG4964190.7877073484062549No Hit
NGTAGTCT4418110.7010565093332767No Hit
AGTAGGCT2692930.4273085336668532No Hit
AGTAGTCG1907490.302676547435019No Hit
CAGTACGG1588440.2520503567555697No Hit
GCAGGAGA1518130.2408937121334976No Hit
ATCCCGAC1501070.23818666680207184No Hit
GTCCCGAC1449000.22992430745814788No Hit
AGTAGACT1427490.2265111453784897No Hit
CAGGACTG1390360.22061943417357524No Hit
CAGAACTG1316550.20890741682817435No Hit
AGTAGCCT1163240.18458050476716079No Hit
CAGTACCG1104170.17520739997657914No Hit
AGTAGTCC992530.15749259688159803No Hit
CAGCACTG769280.12206775102926433No Hit
CAGTACAG662220.10507969281223926No Hit
AGTAGTCA657110.10426884863617913No Hit
AGGAGTCT649720.1030962188003505No Hit
CTCCCGAC632390.10034633043026787No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)