FastQCFastQC Report
Wed 8 May 2024
EGAF00002542503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63348095
Total Bases506.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGAC1596269325.198378893635237No Hit
GCAGTAGA1543693024.368420234262768No Hit
CAGTACTG1403852422.160925281178542No Hit
AGTAGTCT1274264720.115280498963703No Hit
NTCCCGAC6086110.9607408083857929No Hit
NCAGTAGA5768730.9106398542844896No Hit
NAGTACTG5224650.824752504396541No Hit
NGTAGTCT4680780.738898304676723No Hit
AGTAGGCT2186380.3451374504631907No Hit
AGTAGTCG1995740.31504341211839126No Hit
CAGTACGG1521750.24022032548887223No Hit
ATCCCGAC1441640.22757432563678512No Hit
GCAGGAGA1437370.22690027221813064No Hit
GTCCCGAC1427150.22528696403577722No Hit
CAGGACTG1081310.17069337286306716No Hit
CAGAACTG1053200.16625598607187794No Hit
CAGTACCG1042130.16450849863756756No Hit
AGTAGTCC1033890.1632077491833022No Hit
AGTAGACT1033370.16312566305269321No Hit
AGTAGCCT812150.12820432879631188No Hit
AGGAGTCT732220.11558674337405095No Hit
AGTAGTCA708970.11191654618816872No Hit
GCAGTAGG664120.10483661742314429No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)