FastQCFastQC Report
Wed 8 May 2024
EGAF00002542509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62234953
Total Bases497.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGCCT2147425534.505135723328976No Hit
TGTGCGGG1575104525.309001197446072No Hit
ACCATAAC1334602421.44457954358863No Hit
GTGTATTA58928389.46869518805614No Hit
NAACGCCT7999851.2854271778754296No Hit
NGTGCGGG6090700.9786622639531841No Hit
CAACGCCG5774310.9278242726398459No Hit
NCCATAAC5008420.8047599875266235No Hit
CAACGCCC3368850.5413115681151073No Hit
CAACGCCA2468560.39665170149642437No Hit
NTGTATTA2133710.34284753135428575No Hit
GGTGCGGG2017020.32409761762011774No Hit
AGTGCGGG1989900.31973993778062304No Hit
TGAGCGGG1866350.2998877495737805No Hit
TGGGCGGG1709230.27464148643287317No Hit
ACCAGAAC1489280.23929961030098312No Hit
TGCGCGGG1460090.23460931994276593No Hit
GGGTATTA1259030.20230271564598115No Hit
CGTGCGGG1199370.19271646272473283No Hit
GAGTATTA986900.15857648353972403No Hit
ACCACAAC666100.10702988720823811No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)