FastQCFastQC Report
Wed 8 May 2024
EGAF00002542514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48133869
Total Bases385 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATCCG1525494931.69275463811147No Hit
TAATGGGA1064509722.115606372718553No Hit
CCTCATTC1045171421.713845608380243ABI Solid3 Adapter A (100% over 8bp)
GTGGCAAT738791815.348689298173806No Hit
NGCATCCG5444481.1311120658096279No Hit
NAATGGGA3963100.8233495628618593No Hit
NCTCATTC3705840.7699027892397347No Hit
NTGGCAAT2637860.5480257570817754No Hit
AGCAGCCG1743100.36213585905591755No Hit
AGCAACCG1495920.31078324495377674No Hit
GAATGGGA1470590.30552083814413505No Hit
AAATGGGA1347010.27984660863227095No Hit
GTGGCAAG1271560.2641715753204879No Hit
CCTCAGTC1099700.2284669865204478No Hit
AGCACCCG1035790.21518943345277317No Hit
TAAGGGGA929890.19318829325770592No Hit
GTGGCAAA756650.15719700404719178No Hit
CCTCATGC755330.15692276887195586No Hit
CCGCATTC727610.15116383019200055No Hit
CAATGGGA633490.13161003118199371No Hit
CCTCATCC625820.13001655861073624No Hit
CCTCACTC586630.12187468246111692No Hit
CCTCAATC579010.12029159758589114No Hit
TAAAGGGA567870.1179772189100361No Hit
AGCATGCG564080.11718983155083586No Hit
CCCCATTC557710.11586643907640168No Hit
CCACATTC551020.11447656534736488No Hit
GTGGCAAC535260.11120236355818396No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)