FastQCFastQC Report
Thu 9 May 2024
EGAF00002542515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48578035
Total Bases388.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATCCG1555264732.01580096848298No Hit
TAATGGGA1079476222.221487550906495No Hit
CCTCATTC1063948721.90184720316497ABI Solid3 Adapter A (100% over 8bp)
GTGGCAAT749092615.420397305078314No Hit
NGCATCCG5783781.1906162939690748No Hit
NAATGGGA4148530.8539929620455007No Hit
NCTCATTC3924940.8079659870968432No Hit
NTGGCAAT2780530.5723842061540777No Hit
AGCAGCCG1663240.34238519528424727No Hit
GAATGGGA1457790.30009241831210337No Hit
AGCAACCG1416370.2915659309809464No Hit
GTGGCAAG1323730.2724955836521588No Hit
AAATGGGA1299910.2675921329465055No Hit
AGCACCCG1029550.21193734987427962No Hit
CCGCATTC781410.16085665054175205No Hit
GTGGCAAA770690.15864989186985434No Hit
CCTCATGC722460.14872153638985192No Hit
CCTCAGTC720440.14830571059533387No Hit
TAAGGGGA679050.13978539889478855No Hit
CAATGGGA643230.13241169594447366No Hit
CCTCATCC603700.12427427334185091No Hit
CCACATTC596560.12280447325627726No Hit
CCCCATTC590550.12156728859040922No Hit
GTGGCAAC565830.11647856896640632No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)