FastQCFastQC Report
Wed 8 May 2024
EGAF00002542520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60808841
Total Bases486.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA1803795929.663382336131022No Hit
CCAAGATG1340094522.037823414526187No Hit
TACGTGAC1326937521.821456850328723RNA PCR Primer, Index 17 (100% over 8bp)
GTTTATCT1045341617.190618712828286No Hit
NGGCCCGA6554771.0779304279126123No Hit
NACGTGAC4890070.8041708935054361No Hit
NCAAGATG4840000.7959368934527136No Hit
NTTTATCT3578360.5884604838957546No Hit
GGTTATCT2995870.492670136567806No Hit
CCAAGAGG2416370.39737149405626726No Hit
TACGGGAC1548500.254650470973456No Hit
GATTATCT1527500.2511970257745909No Hit
CCAAGACG1501540.24692790970970818No Hit
CCAAGAAG1327440.2182972045134029No Hit
AACGTGAC1265520.20811447466989214TruSeq Adapter, Index 21 (100% over 8bp)
GTGTATCT1194160.19637933898460588No Hit
GCTTATCT1130250.1858693540960598Illumina PCR Primer Index 10 (100% over 8bp)
GACGTGAC1125280.18505203873232842TruSeq Adapter, Index 18 (100% over 8bp)
AGGCCCGG1115070.1833730065665945No Hit
GTTTAGCT958680.15765470682133212No Hit
AGGCCGGA855570.14069829089490457No Hit
AGGCCCGC849950.13977408317977974No Hit
TACGCGAC793810.13054187301481376No Hit
GTTTATCG694240.11416760927905203No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)