FastQCFastQC Report
Wed 8 May 2024
EGAF00002542521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61193052
Total Bases489.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA1822261929.778902023059743No Hit
CCAAGATG1358563622.20127213135243No Hit
TACGTGAC1341295621.919083231867564RNA PCR Primer, Index 17 (100% over 8bp)
GTTTATCT1069954417.48490008310094No Hit
NGGCCCGA6839241.1176497619370251No Hit
NACGTGAC5094590.8325438646204475No Hit
NCAAGATG5084920.8309636198567119No Hit
NTTTATCT3820100.6242702194360236No Hit
GGTTATCT2926310.4782095196036308No Hit
CCAAGAGG2314250.3781883603386868No Hit
TACGGGAC1474240.240916239967897No Hit
GATTATCT1470800.24035408464346572No Hit
CCAAGACG1442650.2357538891833668No Hit
AGGCCCGG1297640.2120567544171518No Hit
CCAAGAAG1264830.20669503459314303No Hit
GTGTATCT1262690.2063453216878282No Hit
AACGTGAC1207940.19739822749811534TruSeq Adapter, Index 21 (100% over 8bp)
GCTTATCT1138340.1860243872131104Illumina PCR Primer Index 10 (100% over 8bp)
GACGTGAC1114100.1820631531828156TruSeq Adapter, Index 18 (100% over 8bp)
AGGCCCGC951420.15547843568907135No Hit
GTTTATCG793150.1296143882478684No Hit
TACGCGAC780120.12748506153933944No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)