FastQCFastQC Report
Wed 8 May 2024
EGAF00002542526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58395047
Total Bases467.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGCCG1739911229.795527007624468No Hit
CCCACAGT1531027426.2184462322635No Hit
TATGATTC1067759618.28510558438287No Hit
ATGCTGAA1039525517.801603961377065No Hit
NGATGCCG6284851.0762642249435983No Hit
NCCACAGT5484880.9392714419769197No Hit
NATGATTC3838520.6573365717130085No Hit
NTGCTGAA3742850.6409533329085256No Hit
CCCACAGG3464710.5933225809373867No Hit
CCCACAGA1933150.3310469122492529No Hit
GGAAGCCG1594380.2730334303866559No Hit
CCCACAGC1591370.27251797571119346No Hit
GGAGGCCG1463970.25070105688929406No Hit
GGACGCCG1095670.1876306392903494No Hit
GATGATTC822460.14084413700360582No Hit
TATGAATC788900.13509707424330014No Hit
TATGAGTC711970.12192301172392242No Hit
ATGCGGAA646610.11073028162816617No Hit
TATGGTTC646220.11066349514197668No Hit
TATGCTTC617870.10580863133820237No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)