FastQCFastQC Report
Wed 8 May 2024
EGAF00002542527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57439829
Total Bases459.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGCCG1708900529.75114184270987No Hit
CCCACAGT1503135826.168876651774152No Hit
TATGATTC1051110018.299323279670627No Hit
ATGCTGAA1022305317.79784720459387No Hit
NGATGCCG6127451.0667597913635851No Hit
NCCACAGT5328580.927680338324127No Hit
NATGATTC3737310.6506478283561742No Hit
CCCACAGG3643910.634387334265915No Hit
NTGCTGAA3636080.6330241686478558No Hit
CCCACAGA2065680.3596250260424696No Hit
CCCACAGC1705060.2968428057123917No Hit
GGAAGCCG1680180.292511316494344No Hit
GGAGGCCG1487400.25894923886350707No Hit
GGACGCCG1110590.19334841682763365No Hit
GATGATTC779270.1356671866136649No Hit
TATGGTTC685030.11926045253372881No Hit
TATGAATC670370.11670821652341619No Hit
ATGCGGAA661910.11523537091309934No Hit
TATGCTTC639480.11133041499827585No Hit
TATGATGC576410.10035022910670573No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)