FastQCFastQC Report
Wed 8 May 2024
EGAF00002542532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57434687
Total Bases459.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGACCT1698876029.57926801272548No Hit
GAGATGAC1595037427.771325714720096No Hit
ACTTCATA1305942622.737872672658597No Hit
TGCCGTGG687299911.96663437897729No Hit
NTAGACCT6127351.066837884917872No Hit
NAGATGAC5694050.9913956700068723No Hit
NCTTCATA4677800.8144555571444134No Hit
NGCCGTGG2520250.43880277435828974No Hit
CTAGACCG2409550.4195287074516485No Hit
GAGAGGAC1631840.28412098772297656No Hit
CTAGACCA1471340.2561762023705292No Hit
CTAGACCC1436240.2500649128635453No Hit
GAGAAGAC1210060.21068452936811513No Hit
GAGACGAC959980.16714289746194666No Hit
TGCCGGGG948530.16514932866265988No Hit
ACGTCATA791020.1377251346385852No Hit
ACTGCATA667060.11614235836263893No Hit
ACTTCAGA658380.11463107651304863No Hit
TGCCGAGG638670.1111993524052808No Hit
ACTCCATA623710.10859465465529568No Hit
TGCCGCGG602140.10483908443690135No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)