FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002542533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56598251
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGACCT1672648329.55300332513809No Hit
GAGATGAC1572385927.781528089975783No Hit
ACTTCATA1286148622.724175699351555No Hit
TGCCGTGG677881911.977082118668296No Hit
NTAGACCT5960221.0530749439589573No Hit
NAGATGAC5546670.9800073150670328No Hit
NCTTCATA4563620.8063182023062868No Hit
CTAGACCG2588850.4574081273288816No Hit
NGCCGTGG2448370.4325875723615558No Hit
CTAGACCA1612200.2848497915598134No Hit
GAGAGGAC1596880.28214299413598487No Hit
CTAGACCC1573910.27808456483929156No Hit
GAGAAGAC1158250.20464413290792327No Hit
GAGACGAC922490.16298913547699556No Hit
TGCCGGGG864960.15282451042524264No Hit
ACGTCATA783300.13839650274705484No Hit
ACTGCATA675910.11942241819451276No Hit
ACTTCAGA663380.11720856886549373No Hit
ACTCCATA615800.10880194866798976No Hit
GAGATGCC590330.10430180960892238Illumina PCR Primer Index 3 (100% over 8bp)
TGCCGAGG570340.10076989834897902No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)