FastQCFastQC Report
Wed 8 May 2024
EGAF00002542538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60878962
Total Bases487 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCCCT1800226429.570583020124424No Hit
GGATGGGC1638905626.92072180862742No Hit
CCGATTAA1277091220.97754557641768No Hit
AATCAATG916593815.056002433155808No Hit
NTCGCCCT6530771.0727466082618162No Hit
NGATGGGC5909920.9707655659437819No Hit
NCGATTAA4567500.7502591782034654No Hit
NATCAATG3294720.5411918816881274No Hit
TTCGCCCG2065280.3392436290224528No Hit
GGAGGGGC1635640.2686708094661666No Hit
CCGAGTAA1535550.25222999038649835No Hit
CCGATGAA1241850.2039867236895399No Hit
TTCGCCCC1163300.19108407268836153No Hit
TTCGCCCA1090180.17907335542284705No Hit
AATCAAGG895170.14704094330649067No Hit
CCGAATAA889380.14608987584249547No Hit
CCGATAAA854170.14030626869098065No Hit
CCGATCAA761400.12506783542071562No Hit
CCGACTAA631670.10375833937510302No Hit
AAGCAATG612970.10068667070900453No Hit
GGACGGGC611930.10051583993826965No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)