FastQCFastQC Report
Wed 8 May 2024
EGAF00002542539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59861485
Total Bases478.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCCCT1767396629.524770392849426No Hit
GGATGGGC1610435226.90269377714235No Hit
CCGATTAA1255369320.9712355114478No Hit
AATCAATG901896715.066393692037543No Hit
NTCGCCCT6337261.0586539909592954No Hit
NGATGGGC5745750.9598408726412316No Hit
NCGATTAA4451310.7436016664137216No Hit
NATCAATG3203510.5351537804316081No Hit
TTCGCCCG2256740.37699365460111794No Hit
GGAGGGGC1655470.2765501056313588No Hit
CCGAGTAA1574290.2629887982230979No Hit
TTCGCCCC1315810.219809114324511No Hit
TTCGCCCA1239770.20710645584552403No Hit
CCGATGAA1080570.18051172636295273No Hit
AATCAAGG897680.14995952739896112No Hit
CCGAATAA895150.14953688502715895No Hit
CCGATAAA719840.12025094265536514No Hit
CCGATCAA657410.10982186626342463No Hit
CCGACTAA631280.10545678911908049No Hit
AAGCAATG614620.10267369745337926No Hit
GGACGGGC609620.10183843584902713No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)