FastQCFastQC Report
Wed 8 May 2024
EGAF00002542544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65748612
Total Bases525.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCGC2051129731.19654754080588No Hit
CCCAATAG1455681222.14010540633162No Hit
TATCGATA1412671721.485954714907137No Hit
GTGTCGCT1139081317.324796149308824No Hit
NGAGTCGC7393101.1244495929434983No Hit
NCCAATAG5244700.7976898432471852No Hit
NATCGATA5134480.7809259912589486No Hit
NTGTCGCT4062070.6178183655040504No Hit
AGAGGCGC3463360.5267578880600552No Hit
AGAGCCGC2114830.32165393848922624No Hit
AGAGACGC1923650.29257651857350236No Hit
GTGTCGCG1452980.22099021649308734No Hit
CCCAAGAG1133450.17239147192947588No Hit
GTGGCGCT1121360.1705526498414902No Hit
GGGTCGCT1048780.15951363353495585No Hit
TATCGAGA963640.14656431074164727No Hit
GTGTCGCC891640.1356135092251073No Hit
TATCGAAA877040.1333929300286978No Hit
GTGTCGCA850550.1293639476374041No Hit
CCCAAAAG811690.12345355670778267No Hit
GATCGATA781490.11886030384945616No Hit
CCCAACAG724290.11016050042242716No Hit
TATCGACA660170.10040820329408626No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)