FastQCFastQC Report
Wed 8 May 2024
EGAF00002542550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60009127
Total Bases480 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGTGC1910500931.83683875287838No Hit
GCGATGTG1442263524.034069017534616No Hit
TTTCCACT1146218419.10073445994307No Hit
AGATACAA1042889417.378846387816974No Hit
NACGGTGC6938721.1562774442627701No Hit
NCGATGTG5147320.857756187654588No Hit
NTTCCACT4198510.6996452389650661No Hit
NGATACAA3769520.628157780065689No Hit
GCGATGGG2096790.3494118486342919No Hit
CACGGGGC1737420.28952595827631356No Hit
GCGAGGTG1457560.24288971909223075No Hit
GCGATGAG1360910.22678383573218786No Hit
GTTCCACT1258370.20969643501062762No Hit
CACGGCGC1180290.19668508092110723No Hit
CACGGAGC1052880.17545331062723174No Hit
GCGATGCG1027310.1711922921324951No Hit
TTTCCACG1023970.17063571013122722No Hit
AGAGACAA919000.15314337100754688No Hit
GCGAAGTG851550.1419034141256546No Hit
GCGACGTG771260.12852378272391798No Hit
ATTCCACT693730.11560408135915724No Hit
TTTCCACA662550.11040820507187182No Hit
TTTCCACC644790.10744865526872271No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)