FastQCFastQC Report
Wed 8 May 2024
EGAF00002542551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59005567
Total Bases472 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGTGC1880141631.863800241085727No Hit
GCGATGTG1415017123.981077921003624No Hit
TTTCCACT1126039219.083609517725677No Hit
AGATACAA1025095617.372862462282583No Hit
NACGGTGC6742101.1426210004896658No Hit
NCGATGTG4988690.8454609037143903No Hit
NTTCCACT4078400.6911890195038716No Hit
NGATACAA3663880.6209380209836811No Hit
GCGATGGG2124340.36002365675089604No Hit
GCGAGGTG1516620.2570299849842982No Hit
CACGGGGC1502840.25469461212024286No Hit
GCGATGAG1374890.233010217493546No Hit
GTTCCACT1205610.20432139903002713No Hit
TTTCCACG1140530.1932919312511648No Hit
GCGATGCG1076270.18240143341051193No Hit
CACGGCGC1024110.17356158953611953No Hit
AGAGACAA921890.1562378004095783No Hit
GCGAAGTG863480.1463387344451753No Hit
CACGGAGC856520.14515918472573952No Hit
GCGACGTG784730.13299253611104184No Hit
TTTCCACA758210.12849804493870892No Hit
TTTCCACC749280.1269846284165018No Hit
ATTCCACT719410.12192239420392317No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)