FastQCFastQC Report
Wed 8 May 2024
EGAF00002542556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63655329
Total Bases509.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCC1700411526.712791006075864No Hit
TTGCCGGA1526543823.981398320948117No Hit
CAAGAATT1338351521.024971844855283No Hit
AGTTGCAG1282989820.15526147072463No Hit
NCCATTCC6171590.9695323387614572No Hit
NTGCCGGA5674730.8914776011918815No Hit
NAAGAATT4872250.7654111724094615No Hit
NGTTGCAG4711340.7401328488931382No Hit
GCCATCCC2116060.33242464271922934No Hit
GCCATGCC1892740.2973419554551356No Hit
GCCAGTCC1746760.2744090757900254No Hit
CAAGAATG1384460.21749318112863733No Hit
CAAGAAGT1367250.2147895583101927No Hit
CAAGAATC1203960.18913734622281192No Hit
AGGTGCAG1036530.16283475653703713No Hit
GCCAATCC964330.151492422574707No Hit
CAAGAATA944880.14843690463056125No Hit
GTGCCGGA913440.14349780518768507No Hit
GCCACTCC881310.1384503094784099No Hit
GCCATACC849110.13339181704645656No Hit
AGTGGCAG831710.13065834598074264No Hit
CAAGAAAT809160.1271158303179927No Hit
TGGCCGGA804670.1264104691062079No Hit
GCCCTTCC724790.11386163757004539No Hit
CAAGAACT702850.1104149504906337No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)