FastQCFastQC Report
Wed 8 May 2024
EGAF00002542557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62622681
Total Bases500.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCC1630802926.041729193932145No Hit
TTGCCGGA1488784623.77388793047682No Hit
CAAGAATT1293701920.658679560525364No Hit
AGTTGCAG1248236819.93266305541917No Hit
NCCATTCC5241460.8369906743532748No Hit
NTGCCGGA4955870.7913857919944373No Hit
NAAGAATT4184280.6681732454092791No Hit
NGTTGCAG4085200.6523515018464316No Hit
GCCAGTCC2607850.4164385743880879No Hit
GCCATCCC2406300.3842537498514316No Hit
GCCATGCC2355290.3761081388387061No Hit
CAAGAAGT1737240.2774138654331966No Hit
CAAGAATG1598020.2551823036768419No Hit
GCCAATCC1570660.25081327961669353No Hit
AGGTGCAG1513950.2417574552581037No Hit
AGTGGCAG1432000.2286711423294062No Hit
GTGCCGGA1326840.21187850453097018No Hit
CAAGAATC1241660.19827640403961627No Hit
GCCACTCC1232070.19674501000683764No Hit
TGGCCGGA1184410.1891343489429972No Hit
CAAGAAAT1148770.183443120233067No Hit
CAAGAATA1111840.17754589587117806No Hit
GCCATACC1040510.16615545412372237No Hit
GCCCTTCC930230.1485452211795276No Hit
GCCGTTCC875170.13975287963158267No Hit
CAAGAACT862710.1377631851948338No Hit
AGTCGCAG759010.12120368976218057No Hit
AGTAGCAG725360.11583023729054334No Hit
TTGCCGGG693260.11070429897436043No Hit
TAGCCGGA653280.10432003062915815No Hit
TTGCCGGC634120.101260436294639No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)