FastQCFastQC Report
Wed 8 May 2024
EGAF00002542562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54919616
Total Bases439.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC2479380945.14563430305121No Hit
CTAGGTGA1343806124.46859970761631RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT1242708722.62777474627645No Hit
NATACGCC9073771.6521910859682631No Hit
NTAGGTGA4895620.8914155554183045No Hit
NGCCAATT4490430.8176368166885944No Hit
CTAGGGGA2338430.42579139664778426No Hit
CGAGGTGA1477820.26908782464902886No Hit
AGCCAAGT1387850.2527056999087539No Hit
AGCCAATG1310210.23856867462438194No Hit
AGCCAATC1158520.21094830670338266No Hit
GAGACGCC1049120.19102828395595484No Hit
TCGTTCAG960080.17481549761746332No Hit
AGCCAATA940150.17118655745881398No Hit
AGCCAAAT850030.1547771200730901No Hit
GAAACGCC842050.15332408733520642No Hit
CTAGGCGA837410.15247921616931917No Hit
CCAGGTGA793410.14446750683762977No Hit
GACACGCC723810.13179443934932103No Hit
AGCCAACT691950.1259932334559659No Hit
CTAGGAGA689740.1255908271463515No Hit
CAAGGTGA684520.12464034708472833Illumina PCR Primer Index 12 (100% over 8bp)
GATACGCG672120.12238250172761586No Hit
GATACGCA614640.11191629599158158No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)