FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002542563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54170151
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC2419430444.663534351233395No Hit
CTAGGTGA1300780924.01287195968865RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT1199861322.149860723112994No Hit
NATACGCC7887791.456113718420316No Hit
NTAGGTGA4160290.7680041357093503No Hit
NGCCAATT3848980.7105352170792362No Hit
CTAGGGGA3151730.5818204198840059No Hit
CGAGGTGA2023540.3735525861834869No Hit
AGCCAAGT1723250.3181179982311661No Hit
GAGACGCC1664780.3073242310142351No Hit
AGCCAATG1494060.275808719824318No Hit
GAAACGCC1354130.24997715070057677No Hit
AGCCAAAT1213860.2240828163835098No Hit
AGCCAATC1196010.220787643733908No Hit
AGCCAATA1054590.19468101538059215No Hit
GACACGCC1005830.18567974824364067No Hit
CAAGGTGA997550.18415123118264892Illumina PCR Primer Index 12 (100% over 8bp)
CTAGGCGA994730.18363064928506476No Hit
CCAGGTGA976690.18030040196860445No Hit
TCGTTCAG934530.1725175179962116No Hit
GATGCGCC922660.17032627433510383No Hit
CTAGGAGA920920.17000506422808384No Hit
AGCCAACT835260.1541919275801908No Hit
GATCCGCC829180.1530695382407186No Hit
GATAGGCC789220.14569278198984528No Hit
GATACGCG762320.14072694757671986No Hit
GATACGCA650760.12013258002548304No Hit
GATAAGCC627780.11589039137070153No Hit
GCTACGCC603360.11138237366183454No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)