FastQCFastQC Report
Wed 8 May 2024
EGAF00002542568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63065707
Total Bases504.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGGCA2203490134.93959244760389No Hit
TGCCTCAT1757362227.865575184941637No Hit
AATAATGG1130924017.932471604575845No Hit
GTGTCATC741141211.751889184402547No Hit
NCAGGGCA8141271.290918692150712No Hit
NGCCTCAT6458821.0241413768658774No Hit
NATAATGG4148520.6578091640199959No Hit
NTGTCATC2661580.4220328490093673No Hit
TGCCTCAG1459020.2313491863335489No Hit
TGCCGCAT1416010.2245293151157411No Hit
AATAAGGG1410380.2236365954004131No Hit
TGCCCCAT1282290.20332603264084553No Hit
TGCCTCAA1111750.17628439494066084No Hit
GTGGCATC1102600.17483352719727696No Hit
GGCCTCAT1044870.1656795824075991No Hit
AAGAATGG961730.15249650654039287No Hit
AAAAATGG842390.1335733856119301No Hit
TGCCTCAC826290.13102049264269724No Hit
CCAGGGCC723240.11468039199179993No Hit
GGGTCATC698440.11074798543049712No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)