FastQCFastQC Report
Wed 8 May 2024
EGAF00002542569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62179702
Total Bases497.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGGCA2176169734.99807220047468No Hit
TGCCTCAT1686385727.121160857284266No Hit
AATAATGG1092902717.57651878100027No Hit
GTGTCATC709690711.413542959726634No Hit
NCAGGGCA7143371.148826670156766No Hit
NGCCTCAT5530490.8894365560002202No Hit
NATAATGG3546930.5704321323379774No Hit
TGCCGCAT2292690.36872000447991854No Hit
NTGTCATC2251620.3621149551343942No Hit
AATAAGGG1937350.31157273799736124No Hit
TGCCCCAT1736410.27925672593284545No Hit
TGCCTCAG1700650.27350565301840785No Hit
GGCCTCAT1557210.2504370316859994No Hit
GTGGCATC1492450.24002205735884677No Hit
AAGAATGG1313320.21121362080506592No Hit
TGCCTCAA1282070.20618786497239885No Hit
TGCCACAT1224990.1970080203986825No Hit
AAAAATGG1191530.19162684311352923No Hit
CCAGGGGA980600.15770419742442637No Hit
GGGTCATC878910.14134998588446115No Hit
TGCCTCAC844940.13588678826411874No Hit
CCAGGGCC789000.12689028326317808No Hit
AGCCTCAT678680.10914815899246348No Hit
AATAACGG657670.10576924283104477No Hit
TGGCTCAT653360.10507609058660332No Hit
CCAGGGCG634730.10207993598940052No Hit
GTGTCAGC627440.10090752766875595No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)