FastQCFastQC Report
Wed 8 May 2024
EGAF00002542574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50959483
Total Bases407.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTACAC1529733230.018616947114634No Hit
ATTGCGTG1328831326.07623197433145No Hit
TACAGACT944745318.539146089845534No Hit
CGACTTGA900197317.66496139688073No Hit
NCGTACAC5583631.0956998916178173No Hit
NTTGCGTG4825960.9470190268610065No Hit
NACAGACT3487110.6842906942364388No Hit
NGACTTGA3283220.6442804767073481No Hit
ATTGCGGG2076400.40746096266322007No Hit
GCGGACAC1938940.3804865916712695No Hit
TACAGACG1238070.2429518368543888No Hit
TACAGACC1058600.20773366166214835No Hit
ATTGCGCG1036210.20333997501505263No Hit
ATTGCGAG960580.18849877264257173No Hit
CGACTGGA857050.16818263246508997No Hit
AGTGCGTG853510.1674879629371436No Hit
TACAGACA852180.1672269712783389No Hit
GACAGACT715390.1403840772874403No Hit
GCGAACAC680080.13345504309767037No Hit
GCGCACAC673190.13210298856446406No Hit
ATGGCGTG595840.11692426314450641No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)