FastQCFastQC Report
Wed 8 May 2024
EGAF00002542575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50280254
Total Bases402.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTACAC1491952329.672727985821233No Hit
ATTGCGTG1281656825.490261047607277No Hit
TACAGACT915472418.20739409948088No Hit
CGACTTGA874277917.38809632902809No Hit
NCGTACAC4820400.9587063740767896No Hit
NTTGCGTG4098460.8151231694255164No Hit
NACAGACT3016650.5999671362042045No Hit
GCGGACAC2931600.583051947191834No Hit
NGACTTGA2826300.5621093322241372No Hit
ATTGCGGG2653290.5277001981732232No Hit
TACAGACG1537850.30585565458758424No Hit
ATTGCGAG1365540.2715857401993236No Hit
GCGAACAC1234350.24549398656577986No Hit
ATTGCGCG1213200.24128756390132794No Hit
TACAGACC1186570.2359912501635334No Hit
CGACTGGA1173510.23339380902888834No Hit
AGTGCGTG1149150.22854896476855505No Hit
TACAGACA1091060.2169957216206585No Hit
GACAGACT1038880.20661789019602012No Hit
GCGCACAC1011150.20110280270262756No Hit
ATGGCGTG870240.17307788461052723No Hit
CGACGTGA800090.1591260855603474No Hit
AATGCGTG651040.12948224167682207No Hit
CGACTCGA619020.12311393653659744No Hit
GCGTGCAC519470.1033149116549809No Hit
ATAGCGTG513990.10222502058163828No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)