FastQCFastQC Report
Wed 8 May 2024
EGAF00002542580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57929108
Total Bases463.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCAC1544024126.653683326178612No Hit
GATCAGCA1433072624.73838540721186No Hit
TGGTGTTT1300531922.450404380471387No Hit
ACAACAGG1030864017.795267967875496No Hit
NTCGTCAC5637940.9732481984704477No Hit
NATCAGCA5264410.9087676613283947No Hit
NGGTGTTT4604760.7948957197821862No Hit
NCAACAGG3785480.6534676832931727No Hit
TGGGGTTT2629220.45386854567137475No Hit
TGGTGGTT2211470.3817545403944421No Hit
CTCGGCAC1702780.29394203687721204No Hit
CGCGTCAC1326980.22906964146591036No Hit
GAACAGCA1215360.209801262605321No Hit
GAGCAGCA1141890.19711851941514444No Hit
GGGTGTTT1101460.19013929922760076No Hit
CTCGCCAC1063220.1835381273262485No Hit
TGGAGTTT1041560.17979907441350557No Hit
TGGTGATT997930.17226745490367296No Hit
TGGTGCTT958280.16542288205093716No Hit
CCCGTCAC911380.15732677948364057No Hit
TGGCGTTT879780.15187183617603778No Hit
TGGTGTGT838530.14475106366215754No Hit
GACCAGCA721060.12447282979050878No Hit
CTCGACAC688160.11879347425822609No Hit
TGGTGTTA685640.1183584597919236No Hit
TGGTGTTG639370.11037111084120266No Hit
CACGTCAC608690.10507498233875792No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)