FastQCFastQC Report
Wed 8 May 2024
EGAF00002542581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56893338
Total Bases455.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCAC1493034126.24268767636731No Hit
GATCAGCA1394101924.50378109296382No Hit
TGGTGTTT1229613421.612607788982253No Hit
ACAACAGG1012986117.805003812572924No Hit
NTCGTCAC4812170.8458231084982217No Hit
NATCAGCA4553440.8003467822541894No Hit
NGGTGTTT3774840.6634942038380662No Hit
NCAACAGG3301110.5802278642887854No Hit
TGGGGTTT3205740.5634649174565922No Hit
CTCGGCAC2593830.45591102423978No Hit
TGGTGGTT2505360.4403608731834297No Hit
CGCGTCAC1794460.31540775477086613No Hit
GAACAGCA1789900.31460625495378736No Hit
GAGCAGCA1617520.2843074526581653No Hit
GGGTGTTT1560190.27423070166844493No Hit
TGGAGTTT1456660.25603349200568964No Hit
CTCGCCAC1431680.25164281976213104No Hit
CTCGACAC1223850.2151130594587366No Hit
TGGTGATT1187100.20865360369609534No Hit
CCCGTCAC1098190.193026114938097No Hit
TGGCGTTT1075230.18899049305210391No Hit
TGGTGTGT1035390.1819879157028895No Hit
TGGTGCTT980550.17234882579749497No Hit
GACCAGCA919250.16157427781790548No Hit
CACGTCAC862670.15162935245599404No Hit
TGGTGTTG673820.11843565937368625No Hit
TGGTGTTA650950.11441585656303027No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)