FastQCFastQC Report
Wed 8 May 2024
EGAF00002542586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84458587
Total Bases675.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGAA2205174926.109540525464865No Hit
AGTCGCTT2037609924.12555043100591No Hit
TCGTCACG1863010722.058274548211422No Hit
GTAATTGC1579669618.703481269465236No Hit
ACGTCACG7221140.8549918079969773No Hit
GCGTCACG6728910.7967111739626902No Hit
TCGGCACG5069920.6002847288932267No Hit
NACGAGAA4397910.5207179229744869No Hit
AGTCGCGT4324360.5120095130173088No Hit
GGAATTGC4080700.48315987100281466No Hit
AGTCGCCT4018750.4758249152333084No Hit
NCGTCACG3711140.43940351500315766No Hit
NGTCGCTT3450570.40855170830646265No Hit
AGTCGCTG3141780.3719905946330833No Hit
NTAATTGC2809270.33262100394836114No Hit
TCGACACG2574150.30478250837892895No Hit
CCGTCACG1817730.2152214552204147No Hit
AGGCGCTT1608910.19049691181785933No Hit
GCAATTGC1536590.1819341353650636No Hit
AGTCGCTC1411180.1670854379792075No Hit
GAAATTGC1292370.15301818866564745No Hit
AGTCGCAT1194320.1414089487431278No Hit
GTAATGGC1009720.11955208296345285No Hit
GTAAGTGC940960.1114108148648047No Hit
CACGAGGA930510.11017352208366925No Hit
AGTCGCTA849210.10054750264765855No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)