FastQCFastQC Report
Wed 8 May 2024
EGAF00002542587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88204195
Total Bases705.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGAA2299071926.065335101125292No Hit
AGTCGCTT2178097424.693807363697385No Hit
TCGTCACG1996871522.63918966665928No Hit
GTAATTGC1674870918.988562845565337No Hit
ACGTCACG6595820.7477898301775783No Hit
GCGTCACG5053560.5729387360771219No Hit
NACGAGAA4316050.48932479912094884No Hit
TCGGCACG4029490.45683654842040106No Hit
NCGTCACG3944620.44721455708540847No Hit
NGTCGCTT3940920.4467950759031359No Hit
AGTCGCCT3393380.3847186633243464No Hit
AGTCGCGT3201190.3629294502375992No Hit
NTAATTGC3088570.35016135003556237No Hit
GGAATTGC3085500.34981329402756867No Hit
AGTCGCTG2653150.3007963510125567No Hit
TCGACACG2264840.2567723677995134No Hit
CCGTCACG1631860.1850093411090028No Hit
AGTCGCTC1347320.1527501044593174No Hit
GCAATTGC1263340.14322901535465518No Hit
AGGCGCTT1154350.13087246020441543No Hit
GAAATTGC969320.10989499989201194No Hit
AGTCGCTA919940.10429662670806077No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)