FastQCFastQC Report
Wed 8 May 2024
EGAF00002542592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87517122
Total Bases700.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGGTC2252601925.73898510967945No Hit
AAGATCAT2095696523.94613136387186No Hit
TGATCTCA1894157121.64327455832014No Hit
CCTGAAGG1738254919.86188371230946No Hit
GTCCGGGC6479960.7404219713714991No Hit
GGATCTCA5189600.592981108313868No Hit
TGAGCTCA4654610.5318513558981064No Hit
AAGATCAG4062030.4641411768545131No Hit
NTCCGGTC4044340.46211985810045264No Hit
GTCCGGCC3836670.43839078711934787No Hit
NAGATCAT3776990.4315715500790805No Hit
AAGAGCAT3478480.39746279590866807No Hit
NCTGAAGG3382360.38647980220373335No Hit
NGATCTCA3307640.3779420443007713No Hit
CCGGAAGG2817510.3219381460007334No Hit
TGAACTCA2682810.3065468720509342No Hit
GGCCGGTC2163680.24722933644915793No Hit
AAGAACAT1839930.21023657519268057No Hit
TGATCCCA1719050.19642442081219258No Hit
CCCGAAGG1563590.17866103960776955No Hit
TGATCGCA1542650.1762683649492039No Hit
AAGATCAC1493630.17066717527571348No Hit
GCCCGGTC1476840.1687486935413621No Hit
GTCCGGAC1448750.16553903589288504No Hit
AAGATCAA1258270.14377415198822466No Hit
CCAGAAGG1211530.13843348276466402No Hit
AAGACCAT1124080.12844115234959394No Hit
TGACCTCA1114900.12739221474856086No Hit
TGATCACA919500.10506515513615725No Hit
GACCGGTC914980.10454868477050695No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)