FastQCFastQC Report
Wed 8 May 2024
EGAF00002542598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86227992
Total Bases689.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTACGC2550395629.577351169211962No Hit
CCCAGGAT2411719327.969099639940588No Hit
TAAGTATG1510940117.522617249396227No Hit
GTTCCTCA1407425216.322138175269117ABI Solid3 Adapter A (100% over 8bp)
CCCAGGAG8061120.934861152744923No Hit
GGTCCTCA5397940.6260078513715128No Hit
AGGGACGC5153010.5976029222621814No Hit
NGGTACGC4928590.5715765710977011No Hit
NCCAGGAT4533860.5257990931761463No Hit
TAAGGATG3438810.3988043696993431No Hit
CCCAGGAA3403730.39473608523784254No Hit
CCCAGGAC3007430.34877653187146No Hit
NAAGTATG2768350.3210500367444484No Hit
AGGAACGC2562090.2971297302156822No Hit
GCTCCTCA2380830.27610871420965016No Hit
NTTCCTCA2280050.2644210942544041No Hit
GAAGTATG2205280.2557498961590106No Hit
AGGCACGC2107480.24440787163407446No Hit
TAAGTAGG2043500.2369880073282931No Hit
GATCCTCA1481580.17182123410690114No Hit
GTGCCTCA1460400.16936495517604075No Hit
GTTCCGCA1397820.1621074511395325No Hit
GGGTACGC1123490.13029295637546565No Hit
GTACCTCA932390.10813077961968544No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)