FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002542599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89967651
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTACGC2668016929.655291322433214No Hit
CCCAGGAT2547045328.310679135103793No Hit
TAAGTATG1612967017.928299584036044No Hit
GTTCCTCA1518387616.87703950389902ABI Solid3 Adapter A (100% over 8bp)
CCCAGGAG6729460.7479866291051659No Hit
NGGTACGC4963810.5517327555878946No Hit
NCCAGGAT4718970.5245185294434329No Hit
GGTCCTCA4337750.48214552139412864No Hit
AGGGACGC4156880.4620416287183045No Hit
NAAGTATG3009570.33451690319223737No Hit
CCCAGGAA2962380.3292716845524843No Hit
TAAGGATG2921590.3247378327127825No Hit
CCCAGGAC2686990.29866179344840293No Hit
NTTCCTCA2676750.2975236065683209No Hit
GCTCCTCA2180420.24235599971371932No Hit
AGGAACGC2020320.22456071460618662No Hit
AGGCACGC1838200.20431788310222748No Hit
TAAGTAGG1457920.16204935705168072No Hit
GAAGTATG1341640.1491247115032491No Hit
GATCCTCA1239400.13776062687242996No Hit
GTGCCTCA1046810.11635404374401195No Hit
GTTCCGCA935650.10399849163562135No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)