FastQCFastQC Report
Wed 8 May 2024
EGAF00002542604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85789852
Total Bases686.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC3206418037.37525972186081No Hit
GAGGATCT2029759223.65966548118069No Hit
CTTATGAG1819248321.205868265164977No Hit
AGACCATA937939610.932990069734588No Hit
NCCTGCGC6286810.7328151119785123No Hit
NAGGATCT3859930.44992850669564044No Hit
NTTATGAG3484630.4061820738424866No Hit
GCCTGCGC3398100.3960957993026961No Hit
GAGGAGCT2972600.34649785851128406No Hit
CGTATGAG2685110.3129869019939561No Hit
CCCTGCGC2618600.3052342367952797No Hit
GAGGATCC2365720.2757575569660617No Hit
TCCGGCGC2277620.2654882770983216No Hit
GAGGAACT2063270.24050280445757152No Hit
GAGGATCG2023500.23586705802919442No Hit
NGACCATA1797940.20957490403410417No Hit
TCCAGCGC1730310.2016916872638969No Hit
AGACCAGA1632060.1902392837791584No Hit
CTGATGAG1491870.17389819019620176No Hit
ACCTGCGC1350000.15736126925594884No Hit
GAGGATCA1337750.15593336144233005No Hit
TCCCGCGC1249530.14565009390621167No Hit
GAGGACCT1148730.13390045246843416No Hit
AGACCAAA1085870.12657324551626456No Hit
AGACCACA925200.10784492319674359No Hit
CTAATGAG910970.10618621885488273No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)